Isolated Heart Perfusion Apparatus
The 1 Liter Reservoir supplies a constant head of perfusate. The perfusate flow rate is controlled by on of two methods. If the reservoir cap is off, the flow rate is controlled directly by a pinch clamp on the outlet tubing. If the reservoir cap is tightly sealed, a mariotte flask is created and the flow rate is set by the perfusion pressure. The height of the reservoir determines this pressure which in turn sets the rate of air allowed to enter the reservoir. As air enters, the perfusate flows out.